Research Article

Maintenance of a Schwann-Like Phenotype in Differentiated Adipose-Derived Stem Cells Requires the Synergistic Action of Multiple Growth Factors

Figure 3

Krox20 gene expression and BDNF production in cells maintained in GGF-2 alone is similar to that of cells in which all stimulating factors have been withdrawn. (a) dASC had a 4.351 ± 0.552-fold increase in the expression of Krox20 (, , ); dASC−/+GGF [0.273 nM] cells had a 4.599 ± 0.568-fold increase (, , ); dASC−/+GGF [2.73 nM] cells a 4.23 ± 0.527-fold increase (, , ); dASC−/+GGF [5.46 nM] cells a 3.657 ± 0.468-fold increase (, , ); and dASC−/+GGF [10.92 nM] cells a 3.648 ± 0.148 (, , ) in Krox20 expression compared to dASC+ cells. There was no significant difference in expression between dASC−/+GGF and dASC. (b) dASC+ secrete 10 ± 0.440 ng/mL BDNF compared to 4.32 ± 0.184 ng/mL in dASC, 3.673 ± 0.314 ng/mL in dASC−/+GGF [0.273 nM], 3.695 ± 0.266 ng/mL in dASC−/+GGF [2.73 nM], 4.351 ± 0.257 ng/mL in dASC−/+GGF [5.46 nM], and 4.243 ± 0.208 ng/mL in dASC−/+GGF [10.92 nM] ( for all experimental groups compared to dASC+, , ).
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