Stemness Characteristics of Periodontal Ligament Stem Cells from Donors and Multiple Sclerosis Patients: A Comparative Study

Figure 6

Neurogenic differentiation potential of hPDLSCs collected from healthy donors and from MS patients. (a) hPDLSCs at P2 (A1) and P15 (B1) and neurogenically induced hPDLSCs at P2 (A2) and P15 (B2) passages positive to βIII-tubulin. (c) MS-hPDLSCs at P2 (C1) and P15 (D1) and neurogenically induced MS-hPDLSCs at P2 (C2) and P15 (D2) passages positive to βIII-tubulin. All differentiated cells displayed different cytoskeleton morphologies detected with actin rearrangement. Mag: 63x. Bar: 10 μm. Neurogenic differentiation capacity was further confirmed by qRT-PCR for neurogenic markers nestin and enolase in P2 and P15 of (b) hPDLSCs ((A3) and (B3), resp.) and (d) MS-hPDLSCs ((C3) and (D3), resp.). . Data are expressed as mean ± SEM.