Research Article

Soft Tissue Repair with Easy-Accessible Autologous Newborn Placenta or Umbilical Cord Blood in Severe Malformations: A Primary Evaluation

Figure 5

Morphology and protein expression in PRGF-SIS constructs. The PRGF-SIS constructs were kept in culture for up to 14 days and thereafter analyzed. The HTX/eosin staining showed decreasing number of cells and a complete degradation of the gel at day 14. The cells expressed CD45 (brown staining), and few CD34-positive cells were detected throughout the time points. Ki67-positive cells were detected, but with decreasing number over time. The caspase 3 expression was low but increased over time. Single positive cells stain brown as indicated by the arrows. Magnification 2x and 60x.