Adipose-Derived Stem Cells Cocultured with Chondrocytes Promote the Proliferation of Chondrocytes

Figure 7

Morphology ((a)–(c)) and apoptosis assay ((d)–(i)) of chondrocytes cultured with control medium ((a), (d), and (g)), ADSCs cocultured system ((b), (e), and (h)), and TGF-β1-containing medium ((c), (f), and (i)) for 5 days. Morphology assay showed some membrane blebbing in the chondrocytes cultured with control medium (a), and the cell blebbing numbers were decreased in chondrocytes cultured with ADSCs cocultured system (b) and TGF-β1-containing medium (c). Hoechst 33258 staining showed many chromatin condensations in chondrocytes cultured with control medium ((d), (g)), and much less apoptosis of cells was found in chondrocytes cultured with ADSCs cocultured system ((e), (h)) and TGF-β1-containing medium ((f), (i)). The images of (g), (h), and (i) were the corresponding higher magnification images of (d), (e), and (f), respectively.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)