Allogeneic Umbilical Cord-Derived Mesenchymal Stem Cells as a Potential Source for Cartilage and Bone Regeneration: An In Vitro Study

Figure 2

Chondrogenic differentiation of UC-MSCs seeded on a scaffold. (a) A representative image of safranin o staining of UC-MSCs seeded onto Chondro-gide (A and B) or Hyaff (C and D) membranes and cultured in normoxic (A and C) or low oxygen tension conditions (B and D). UC-MSCs differentiate inside the three-dimensional structure of the HYAFF-11, while they are confined to the surface of the Chondro-gide scaffold. A stronger safranin o staining is observed in the low oxygen tension constructs than in normoxic culture. Magnification 20x. Scale bar: 200 μm in (A and B); 100 μm in (C and D). (b) No significant difference in cell number per field is observed comparing normoxic and in low oxygen tension conditions in Chondro-gide (A) (median value and standard deviation: 174 ± 32.07; 157.5 ± 40.32 for low oxygen tension and normoxia, resp.) and Hyaff (B) (median value and standard deviation: 82.50 ± 24.96; 110 ± 6952 for low oxygen tension and normoxia, resp.) scaffolds. value > 0.05; n of samples = 3.

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