Allogeneic Umbilical Cord-Derived Mesenchymal Stem Cells as a Potential Source for Cartilage and Bone Regeneration: An In Vitro Study

Figure 3

Chondrogenic differentiation of UC-MSCs seeded on Chondro-gide. (a) A representative image of immunofluorescence staining for sox-9 in Chondro-gide scaffold at low oxygen tension (A) and normoxic (B) conditions. Quantification of the signal is shown in (C): Fluorescence intensity is significantly higher in constructs grown at low oxygen tension (median value and standard deviation: 20 ± 6122; 11 ± 2102 for low oxygen tension and normoxia, resp.). value < 0.05. Magnification 20x; n of samples = 3. (b) A representative image of immunofluorescence staining for collagen type II in Chondro-gide scaffold at low oxygen tension (A) and normoxic (B) conditions. Signal quantification is shown in (C); no significant difference in fluorescence intensity is observed (median value and standard deviation: 17 ± 3279; 17 ± 4025 for low oxygen tension and normoxia, resp.). value = 0.6926. Magnification 20x; n of samples = 3.

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