TY - JOUR A2 - Chen, Yanfang AU - Chen, Zhong-Yan AU - Chen, Fei AU - Cao, Nan AU - Zhou, Zhi-Wen AU - Yang, Huang-Tian PY - 2017 DA - 2017/06/05 TI - miR-142-3p Contributes to Early Cardiac Fate Decision of Embryonic Stem Cells SP - 1769298 VL - 2017 AB - MicroRNAs (miRNAs) play important roles in cell fate decisions. However, the miRNAs and their targets involved in the regulation of cardiac lineage specification are largely unexplored. Here, we report novel functions of miR-142-3p in the regulation of cardiomyocyte differentiation from mouse embryonic stem cells (mESCs). With a miRNA array screen, we identified a number of miRNAs significantly changed during mESC differentiation into the mesodermal and cardiac progenitor cells, and miR-142-3p was one among the markedly downregulated miRNAs. Ectopic expression and inhibition of miR-142-3p did not alter the characteristics of undifferentiated ESCs, whereas ectopic expression of miR-142-3p impaired cardiomyocyte formation. In addition, ectopic expression of miR-142-3p inhibited the expression of a cardiac mesodermal marker gene Mesp1 and downstream cardiac transcription factors Nkx2.5, Tbx5, and Mef2c but not the expression of three germ layer-specific genes. We further demonstrated that miR-142-3p targeted the 3′-untranslated region of Mef2c. These results reveal miR-142-3p as an important regulator of early cardiomyocyte differentiation. Our findings provide new knowledge for further understanding of roles and mechanisms of miRNAs as critical regulators of cardiomyocyte differentiation. SN - 1687-966X UR - https://doi.org/10.1155/2017/1769298 DO - 10.1155/2017/1769298 JF - Stem Cells International PB - Hindawi KW - ER -