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Stem Cells International
Volume 2017, Article ID 1960965, 18 pages
Research Article

Characterization of Mesenchymal Stem Cell-Like Cells Derived From Human iPSCs via Neural Crest Development and Their Application for Osteochondral Repair

1Graduate School of Medicine, Orthopaedic Surgery, Osaka University, Suita, Osaka, Japan
2Graduate School of Frontier Bio Science, Orthopaedic Surgery, Osaka University, Suita, Osaka, Japan
3Center for iPS Cell Research and Application, Life Science Frontiers, Kyota University, Kyoto, Japan
4Graduate School of Medicine, Orthopaedic Surgery, Sapporo Medical University, Sapporo, Hokkaido, Japan
5McCaig Institute for Bone and Joint Health, University of Calgary, Calgary, AB, Canada
6Institute for Medical Science in Sports, Osaka Health Science University, Osaka, Japan
7Grobal Center for Medical Engineering and Informatics, Osaka University, Suita, Osaka, Japan

Correspondence should be addressed to Norimasa Nakamura;

Received 20 December 2016; Accepted 3 April 2017; Published 18 May 2017

Academic Editor: Andrzej Lange

Copyright © 2017 Ryota Chijimatsu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Mesenchymal stem cells (MSCs) derived from induced pluripotent stem cells (iPSCs) are a promising cell source for the repair of skeletal disorders. Recently, neural crest cells (NCCs) were reported to be effective for inducing mesenchymal progenitors, which have potential to differentiate into osteochondral lineages. Our aim was to investigate the feasibility of MSC-like cells originated from iPSCs via NCCs for osteochondral repair. Initially, MSC-like cells derived from iPSC-NCCs (iNCCs) were generated and characterized in vitro. These iNCC-derived MSC-like cells (iNCMSCs) exhibited a homogenous population and potential for osteochondral differentiation. No upregulation of pluripotent markers was detected during culture. Second, we implanted iNCMSC-derived tissue-engineered constructs into rat osteochondral defects without any preinduction for specific differentiation lineages. The implanted cells remained alive at the implanted site, whereas they failed to repair the defects, with only scarce development of osteochondral tissue in vivo. With regard to tumorigenesis, the implanted cells gradually disappeared and no malignant cells were detected throughout the 2-month follow-up. While this study did not show that iNCMSCs have efficacy for repair of osteochondral defects when implanted under undifferentiated conditions, iNCMSCs exhibited good chondrogenic potential in vitro under appropriate conditions. With further optimization, iNCMSCs may be a new source for tissue engineering of cartilage.