Crosstalk with Inflammatory Macrophages Shapes the Regulatory Properties of Multipotent Adult Progenitor Cells

Figure 1

rMAPC increase NO production in the presence of inflammatory macrophages. (a) Direct contact cocultures () of macrophages with rMAPC in increasing ratios (1 : 0 to 1 : 4) and (b) transwell cocultures of macrophages with rMAPC (1 : 4, ), supplemented with LPS. The results are shown as percentage of nitrite levels normalized to the positive control (1 : 0 +LPS) with duplicates per experiment. Asterisks () indicate statistical significant difference with positive control (1 : 0 +LPS). (c) Schematic illustration of generation of LPS-activated SN (MΦ; macrophages). (d) rMAPC treated with SN of LPS-activated macrophages, SN of naïve macrophages or LPS alone (). The results are shown as percentage of nitrite levels normalized to the positive control (pure s/n from activated macrophages, dotted line). Asterisks () indicate statistical significant difference with positive control. (e) iNOS mRNA expression of rMAPC treated with SN of LPS-activated macrophages, SN of naïve macrophages or LPS (). The results are shown as fold differences in comparison to SN of naïve macrophages. Asterisks () indicate statistical significant differences. Mean values ± SEM. Data were analyzed with one-way ANOVA followed by Dunnett’s multiple comparison test or with Kruskal-Wallis test followed by Dunn’s multiple comparison test for nonparametric dat ( and ).

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