Review Article
A Concise Review on the Use of Mesenchymal Stem Cells in Cell Sheet-Based Tissue Engineering with Special Emphasis on Bone Tissue Regeneration
Table 1
Primers for reverse transcription polymerase chain reaction (RT-PCR) analysis of gene expression.
| | Gene | GeneBank Acc. number | Primer sequence (5′-3′) |
| | GAPDH | NM_017008.3 | F | GGGCTCTCTGCTCCTCCCTGT | | R | CATGGGGGCATCAGCGGAAGG | | Collagen type I, alpha 2 | NM_053356.1 | F | AGCAGGTCCCCGAGGCAGAG | | R | GCAGGACCCGTTTGTCCGGG | | Osteopontin | AB001382.1 | F | TTGCCTGTTCGGCCTTGCCT | | R | ACGCTGGGCAACTGGGATGA | | Alkaline phosphatase | NM_013059.1 | F | CGGGTGAACCACGCCACTCC | | R | GGCCAGCAGTTCAGTGCGGT | | Osteocalcin | M25490.1 | F | TCCTGGGGTTTGGCTCCTGCT | | R | GGCGAAGGCCTGGAAGGGGA |
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F: forward primer; R: reverse primer.
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