Research Article

Pulsed Electromagnetic Field Regulates MicroRNA 21 Expression to Activate TGF-β Signaling in Human Bone Marrow Stromal Cells to Enhance Osteoblast Differentiation

Table 2

Genes regulated by PEMF during hBMSCs proliferation by microarray analysis. Cells were from a normal 27-year-old female. Total RNA was isolated at day 5 after 2 h of PEMF treatment and used for microarray assays as described in Materials and Methods. Analysis by Student’s -test.

Gene symbolGene titleFold-change
(Avg PEMF versus avg controls)
p

Cell adhesion and binding and cytoskeletal and structural proteins
MMP1Matrix metallopeptidase 1 (interstitial collagenase)
PRC1Protein regulator of cytokinesis 1
CCBE1Collagen and calcium binding EGF domains 1
CLDN1Claudin 1
CENPKCentromere protein K
GAS2L3Growth arrest-specific 2 like 3
CLDN11Claudin 11
NUSAP1Nucleolar and spindle associated protein 1
COL15A1Collagen, type XV, alpha 1
HAPLN1Hyaluronan and proteoglycan link protein 1
IBSPIntegrin-binding sialoprotein
FBN2Fibrillin 2
COL14A1Collagen, type XIV, alpha 1
MMP12Matrix metallopeptidase 12 (macrophage elastase)
MGPMatrix Gla protein

p53 signaling pathway, apoptosis, and survival antiapoptotic TNFs/NF-kB/IAP pathway
BIRC5Baculoviral IAP repeat containing 5
GTSE1G-2 and S-phase expressed 1
SESN3sestrin 3

Cell cycle role of APC  (anaphase-promoting complex) in cell cycle regulation, cell cycle/checkpoint control
CDK1Cyclin-dependent kinase 1
CDC20Cell division cycle 20 homolog (S. cerevisiae)
CCNB2Cyclin B2
NDC80NDC80 kinetochore complex component homolog (S. cerevisiae)
TYMSThymidylate synthetase
CCNB1Cyclin B1
NEK2NIMA- (never in mitosis gene a-) related kinase 2
CCNA2Cyclin A2
TTKTTK protein kinase

Akt signaling
CCL2Chemokine (C-C motif) ligand 2
CSF2RBColony stimulating factor 2 receptor, beta, low-affinity

Other receptor, kinase, and regulator
CDKN3Cyclin-dependent kinase inhibitor 3
PDGFRAPlatelet-derived growth factor receptor, alpha polypeptide
CTSCCathepsin C
LEPRLeptin receptor
FGFR2Fibroblast growth factor receptor 2