Table of Contents Author Guidelines Submit a Manuscript
Stem Cells International
Volume 2017, Article ID 2451927, 18 pages
https://doi.org/10.1155/2017/2451927
Research Article

Effects of Aminoglycoside Antibiotics on Human Embryonic Stem Cell Viability during Differentiation In Vitro

1Department of Biochemistry, College of Medicine and Health Sciences, UAE University, Al Ain, Abu Dhabi, UAE
2Department of Anatomy, College of Medicine and Health Sciences, UAE University, Al Ain, Abu Dhabi, UAE

Correspondence should be addressed to Suraiya A. Ansari; ea.ca.ueau@irasnas

Received 6 April 2017; Revised 30 July 2017; Accepted 29 August 2017; Published 24 September 2017

Academic Editor: Heinrich Sauer

Copyright © 2017 Divya S. Varghese et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Figure S1. Antibiotic treatment of H9 cells during hepatic and neural differentiation causes cell death. The cells were treated with 0, 10, 25, 50 and 200ug/ml gentamicin (images 1-5 respectively) and 0, 10, 25, 50 and 200ug/ml gentamicin combined with Pen-Strep (100U/ml-100ug/ml) images 6-10 respectively. The cells were imaged in bright-field on day-5 and day-10 of hepatic and neural differentiation respectively. Figure S2. RNA expression analysis of indicated genes from H9 cells differentiated towards neurons. Number of reads from RNA-seq data for indicated genes were retrieved from CORTECON (http://cortecon.neuralsci.org/) [13] and were plotted against days post neural induction. The data are represented as mean ± standard deviation.

  1. Supplementary Material