Research Article

Human Adipose-Derived Stem Cells Exhibit Enhanced Proliferative Capacity and Retain Multipotency Longer than Donor-Matched Bone Marrow Mesenchymal Stem Cells during Expansion In Vitro

Figure 2

Population kinetics. (a) Representative phase-contrast microscopy images of donor-matched MSCs and ASCs during monolayer culture at ELSC and LLSC. (b) Cumulative population doublings over time in culture demonstrating enhanced proliferation rate in ASCs compared to donor-matched MSCs (). Points represent ELSC, MLSC, and LLSC, ±SEM for both CPD and days in culture. (c) Mean telomere length in donor-matched MSCs and ASCs at ELSC, MLSC, and LLSC (±SEM) demonstrating longer telomeres in ASCs compared to MSCs and a decrease in mean telomere length with time in culture in both cell types (). represents at MLSC or LLSC compared to ELSC. + represents in ASCs compared to MSCs at the same LSC.
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