Human Adipose-Derived Stem Cells Exhibit Enhanced Proliferative Capacity and Retain Multipotency Longer than Donor-Matched Bone Marrow Mesenchymal Stem Cells during Expansion In Vitro

Figure 4

Assessment of the influence of monolayer expansion of the chondrogenic potential of donor-matched MSCs and ASCs. (a) Quantitative real-time PCR analysis of chondrogenic marker genes SOX-9, COL2A1, ACAN, and COL10A1 at ELSC, MLSC, and LLSC in MSCs and ASCs following pellet culture for 21 days in chondrogenic media. Data was normalised to the mean of the reference genes MRPL19 and EIF2B1 and presented as relative fold change in gene expression compared to day 0 controls plotted on a log scale. . Values are mean ± SEM. . (b) DMMB analysis of GAG synthesis in MSCs and ASCs at ELSC and LLSC. Data represents mean GAG concentration from two pellets for each cell sample (μg/μg DNA) ± SEM. . compared to day 0 control. (c) Representative histological images of ELSC and LLSC in MSC and ASC pellets following 21 days in culture in a chondrogenic media. All images taken at equivalent magnification to allow comparison of size of pellets.

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