Research Article
The Role of Nephronectin on Proliferation and Differentiation in Human Dental Pulp Stem Cells
Table 1
Primer sequence, fragment size, and annealing temperature.
| | Gene name | Forward | Backward | Fragment size (bp) | Annealing temperature (°C) |
| | hGAPDH (NM_001289746.1) | CACTAGGCGCTCACTGTTCTCT | CGTTCTCAGCCTTGACGGT | 250 | 66 | | hDSPP (NM_014208.3) | TGCTGGCCTGGATAATTCCG | CTCCTGGCCCTTGCTGTTAT | 136 | 66 | | hBSP (NM_004967.3) | AAGGGCACCTCGAAGACAAC | CCCTCGTATTCAACGGTGGT | 119 | 62.8 | | hITGA1 (NM_181501.1) | CTCACTGTTGTTCTACGCTGC | ACGACTTGAAATGTGGGGCT | 419 | 59.9 | | hITGA2 (NM_002203.3) | GTGGCTTTCCTGAGAACCGA | GAAGCTGGCTGAGAGCTGAA | 278 | 62.8 | | hITGA3 (NM_002204.3) | ATGGCAAGTGGCTGCTGTAT | GCACTCTAGCCACACACAGT | 272 | 59.9 | | hITGA4 (NM_000885.5) | AATCCCGGGGCGATTTACAG | TCCAGCTTGACATGATGCAAAA | 354 | 59.9 | | hITGA5 (NM_002205.4) | CCCTCATCTCCGGGACACTA | ATCCAACTCCAGGCCCTTTG | 397 | 56.3 | | hITGA6 (NM_000210.3) | CTCGCTGGGATCTTGATGCT | TCAGATGGCTGAGCATGGAT | 128 | 59.9 | | hITGA7 (NM_002206.2) | GGAAGACCGACAGCAGTTCA | ATCTTGATGCGACACCAGCA | 264 | 59.9 | | hITGA8 (NM_003638.2) | GCCTATGCCGAGTTCTCTCC | CCCAGTAAACTCCCCAGCAG | 297 | 59.9 | | hITGB1 (NM_002211.3) | GCCGCGCGGAAAAGATGAAT | TGCTGTTCCTTTGCTACGGT | 323 | 59.9 | | hITGB3 (NM_000212.2) | GAAGCAGAGTGTGTCACGGA | ACATGACACTGCCCGTCATT | 201 | 59.9 |
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