Immunoprofiling of Adult-Derived Human Liver Stem/Progenitor Cells: Impact of Hepatogenic Differentiation and Inflammation

Table 1

Conjugated monoclonal antibodies used for flow cytometry analysis.

Primary antibody	Order ID	Species	Dilution	Source
Endothelial, stromal, and embryonic markers
Anti-CD34-PC5	555823	Mouse	1/20	BD
Anti-CD90-PE	FAB2067P	Mouse	1/20	R&D
Anti-CD105-FITC	326040	Mouse	1/20	AC
Anti-SSEA4-PE	FAB1435P	Mouse	1/20	R&D
Receptors
Anti-CD45-PC7	557748	Mouse	1/20	BD
Anti-CD95-FITC	130092415	Mouse	1/20	MB
Anti-CD184-PE	555974	Mouse	1/20	BD
Anti-CD200R-PE	329306	Mouse	1/20	BL
Anti-CD210-PE	556013	Rat	1/20	BD
Anti-CD271-PE	120002227	Mouse	1/20	MB
Anti-CD229-PE	326108	Mouse	1/20	BL
Human leukocyte antigens
Anti-HLA-ABC-PE-Cy5	15998342	Mouse	1/20	EB
Anti-HLA-DR-PerCP	347402	Mouse	1/20	BD
Anti-HLA-G-PE	1P292C100	Mouse	1/20	ExBIO
Costimulatory molecules
Anti-CD27-APC-Cy7	302815	Mouse	1/20	BL
Anti-CD40-PE	130094135	Mouse	1/20	MB
Anti-CD70-PE	355104	Mouse	1/20	BL
Anti-CD80-FITC	11080942	Mouse	1/20	EB
Anti-CD86-APC	130094876	Mouse	1/20	MB
Anti-CD134-FITC	350006	Mouse	1/20	BL
Anti-CD154-PE-Cy5	310808	Mouse	1/20	BL
Anti-CD252-PE	326308	Mouse	1/20	BL
Cell adhesion molecules
Anti-CD29-PE-Cy5	559882	Mouse	1/20	BD
Anti-CD31-PE	130092653	Mouse	1/20	MB
Anti-CD44-FITC	130095195	Mouse	1/20	MB
Anti-CD49e-PE	555617	Mouse	1/20	BD
Anti-CD54-PE	555511	Mouse	1/20	BD
Anti-CD58-FITC	555920	Mouse	1/20	BD
Anti-CD62e-fluorescein	BBA21	Mouse	1/20	R&D
Anti-CD102-FITC	328507	Mouse	1/20	BL
Anti-CD106-PE-Cy5	551148	Mouse	1/20	BD
Anti-CD146-PC5	A22364	Mouse	1/20	BC
Anti-CD166-PE	559263	Mouse	1/20	BD
Immunoregulatory molecules
Anti-CD39-FITC	328205	Mouse	1/20	BL
Anti-CD73-PE	344003	Mouse	1/20	BD
Anti-CD200-APC	329208	Mouse	1/20	BL
Anti-CD274-PE	557924	Mouse	1/20	BD
Anti-HO-1-PE	ADI-OSA-111	Mouse	1/20	ELS
NK ligands
Anti-CD112-PE	337410	Mouse	1/20	BL
Anti-CD155-PE	337508	Mouse	1/20	BL
Anti-ULBP-3-PE	FAB1517P	Mouse	1/20	R&D