Immunoprofiling of Adult-Derived Human Liver Stem/Progenitor Cells: Impact of Hepatogenic Differentiation and Inflammation

Table 2

Expression levels of different molecules present on undifferentiated and differentiated ADHLSC as well as hepatocytes being cultivated in the presence or absence of inflammation cocktail. The data are presented as the mean ± SEM (standard error of the mean) percentage of each marker expression.

	ADHLSCs				Hepatocytes
	Undifferentiated		Differentiated
	Constitutive	Priming	Constitutive	Priming	Constitutive	Priming
Endothelial, stromal, and embryonic markers
CD34	0,96 ± 0,28	0,54 ± 0,17	0,37 ± 0,15	0,9 ± 0,28	3,5 ± 0,64	1,33 ± 0,92
CD90	82 ± 3,24	84 ± 2,52	75,20 ± 7,80	78,50 ± 8,36	0,32 ± 0,15	0,34 ± 0,22
CD105	31,83 ± 3,63	26 ± 2,63	29,45 ± 3,46	28,66 ± 3,47	0,40 ± 0,20	0,1 ± 0,04
SSEA4	1,4 ± 0,38	2,52 ± 0,40	1,62 ± 0,31	1,98 ± 0,50	0,57 ± 0,19	1,96 ± 0,52
Receptors
CD45	0,78 ± 0,23	1,29 ± 0,46	1,38 ± 0,60	0,71 ± 0,18	0,12 ± 0,08	2,08 ± 1,69
CD95	2,45 ± 0,58	6,80 ± 1,94	3,25 ± 1,14	7,34 ± 1,92	0,20 ± 0,17	2 ± 0,86
CD184	1,53 ± 0,55	2,06 ± 0,68	1,13 ± 0,18	1,06 ± 0,20	0,49 ± 0,23	0,73 ± 0,15
CD200R	1,02 ± 0,27	1,23 ± 0,12	0,79 ± 0,08	1,13 ± 0,33	0,32 ± 0,08	0,69 ± 0,29
CD210	0,73 ± 0,19	1,6 ± 0,10	1,23 ± 0,13	1,30 ± 0,27	0,30 ± 0,18	0,66 ± 0,24
CD271	2,18 ± 0,79	2,20 ± 0,34	2,33 ± 0,61	2,80 ± 0,60	0,42 ± 0,18	1,36 ± 0,72
CD229	0,66 ± 0,17	0,53 ± 0,13	0,34 ± 0,09	0,47 ± 0,14	0,28 ± 0,08	0,25 ± 0,03
Human leukocyte antigens
HLA-ABC	92,83 ± 2,61	93,9 ± 2,88	96,16 ± 1,47	98,2 ± 0,34	89 ± 2,97	88 ± 5,02
HLA-DR	0,78 ± 0,28	0,66 ± 0,23	0,6 ± 0,33	0,4 ± 0,15	1,5 ± 0,3	1,52 ± 1,49
HLA-G	2,43 ± 1,13	1,62 ± 0,37	1,57 ± 0,28	1,58 ± 0,6	0,29 ± 0,16	0,47 ± 0,13
Costimulatory molecules
CD27	0,27 ± 0,12	0,57 ± 0,20	1,06 ± 0,34	0,3 ± 0,12	0,36 ± 0,1	0,5 ± 0,07
CD40	8,33 ± 0,88	70 ± 5,70	9,33 ± 0,84	68,6 ± 4,84	3,5 ± 0,65	72,8 ± 5,22
CD70	0,84 ± 0,22	0,87 ± 0,27	0,79 ± 0,30	0,57 ± 0,15	0,14 ± 0,06	0,71 ± 0,20
CD80	1,13 ± 0,34	1,66 ± 0,77	1,06 ± 0,60	1,44 ± 0,83	1,43 ± 0,07	1,53 ± 0,05
CD86	0,75 ± 0,18	0,67 ± 0,31	0,76 ± 0,34	1,18 ± 0,44	2,34 ± 1,02	1,05 ± 0,84
CD134	1,56 ± 0,60	1,30 ± 0,75	1,66 ± 0,59	1,58 ± 0,67	0,26 ± 0,10	0,13 ± 0,05
CD154	0,42 ± 0,12	1,03 ± 0,33	2 ± 0,40	1,36 ± 0,33	0,45 ± 0,10	0,25 ± 0,10
CD252	5 ± 1,06	4,65 ± 0,20	3 ± 0,57	4,60 ± 0,71	0,43 ± 0,15	0,52 ± 0,10
Cell adhesion molecules
CD29	91 ± 4,80	89,60 ± 4,94	95 ± 1,38	97 ± 0,65	84 ± 3	89 ± 3,82
CD31	1,2 ± 0,45	1,7 ± 0,64	1,3 ± 0,66	1,6 ± 0,93	0,26 ± 0,11	0,31 ± 0,13
CD44	92,83 ± 1,64	93,60 ± 2,26	59,50 ± 6,23	89,60 ± 3,89	57,43 ± 2,40	53,75 ± 4,53
CD49e	82 ± 5,43	89,75 ± 3,87	92,98 ± 3,45	97,23 ± 0,51	38,75 ± 7,22	34,33 ± 5,29
CD54	69 ± 5,53	97,80 ± 0,67	64,20 ± 5,85	88,80 ± 4,92	63,25 ± 9	85,50 ± 5,90
CD58	3,12 ± 1,17	7,44 ± 2,74	2,68 ± 1,47	3,06 ± 1,42	0,80 ± 0,45	3,50 ± 0,65
CD62e	1,96 ± 0,52	2,4 ± 0,98	1,56 ± 0,57	3,03 ± 1,63	0,2 ± 0,09	0,9 ± 0,42
CD102	1 ± 0,25	0,98 ± 0,56	0,88 ± 0,30	1,40 ± 0,72	0,1 ± 0,04	0,1 ± 0,01
CD106	8,08 ± 1,58	48,75 ± 5,54	1,09 ± 0,82	49,50 ± 5,10	0,43 ± 0,02	7,75 ± 4,70
CD146	4,83 ± 1,51	3,64 ± 0,82	3,16 ± 0,60	4 ± 0,64	0,1 ± 0,033	0,04 ± 0,038
CD166	25,60 ± 1,64	24,4 ± 1,31	26,83 ± 2,03	27,20 ± 3,71	30,93 ± 0,05	31,67 ± 3,50
Immunoregulatory molecules
CD39	1,55 ± 0,54	1,48 ± 0,64	2,68 ± 1,29	2,52 ± 1,26	0,03 ± 0,02	0,09 ± 0,01
CD73	88,8 ± 3,99	90 ± 2,33	71,8 ± 6,72	79,75 ± 5,79	2,01 ± 0,08	1,8 ± 0,52
CD200	69,20 ± 2,96	60 ± 8,05	2,43 ± 1,19	1,86 ± 1,05	0,14 ± 0,08	0,61 ± 0,47
CD274	1,50 ± 0,35	82,40 ± 5,94	0,76 ± 0,13	64,80 ± 2,26	0,60 ± 0,34	66,25 ± 2,98
HO-1	65,83 ± 2,21	66,25 ± 3,10	61,83 ± 4,78	68,60 ± 4,22	32,55 ± 5,10	35,66 ± 2,02
NK ligands
CD112	50 ± 5,82	58,75 ± 4,30	46,08 ± 3,35	43,60 ± 5,24	23,5 ± 1,55	25,55 ± 1,93
CD155	71,60 ± 4,32	70,50 ± 2,63	72,80 ± 4,41	74 ± 6,55	33,75 ± 2,43	37,25 ± 3,01
ULBP-3	1,07 ± 0,26	1,46 ± 0,42	0,72 ± 0,16	1,20 ± 0,52	0,53 ± 0,25	1 ± 0,21