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Stem Cells International
Volume 2017 (2017), Article ID 2740272, 9 pages
Research Article

Identification of Stem Leydig Cells Derived from Pig Testicular Interstitium

College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China

Correspondence should be addressed to Chuanying Pan; moc.621@napgniynauhc

Received 26 August 2016; Accepted 19 December 2016; Published 24 January 2017

Academic Editor: James Adjaye

Copyright © 2017 Shuai Yu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

EDS was used to eliminate differentiated LCs in the isolated primary cells in this study. As shown in Figure S1, a part of primary cells dead when incubated with different concentrations of EDS (0, 0.5, 0.75, and 1.0 mg/mL). The dead cell percentage was approximately 23%, which showed that the percentage of differentiated LCs was approximately 23%, and the purity of primary isolated porcine SLCs was over 77% (Figure S1, S2). Then qRT-PCR results showed that the expressions of Nestin and CYP17A1 were statistically significance in 1.0 mg/mL EDS treated group than control group, which predicted that 1.0 mg/mL was the best treatment concentration of EDS in this study (Figure S3). At the foundation of this result, immunofluorescent analysis was used to detected the expression of CYP17A1 in the primary cells before and post EDS treatment. As shown in Figure S4, the percentage of CYP17A1-positive cells was approximately 25% before EDS treatment, which was consistent with the dead cell percentage. Meanwhile, the membrane structures of CYP17A1-positive cells were broke after EDS treatment, predicting that EDS could be eliminate porcine differentiated LCs in this study (Figure S4).

  1. Supplementary Material