Paracrine Secreted Frizzled-Related Protein 4 Inhibits Melanocytes Differentiation in Hair Follicle

Figure 4

Immunofluorescence staining shows proliferation and differentiation of melanocytes after sFRP4 treatment. (a) Double staining shows colocalization of PCNA and TRP2 (white arrow, PCNA), and statistical chart reveals PCNA-positive proliferative cells in the hair follicles after sFRP4 or PBS treatment. (b) Double staining shows colocalization of TRP1 and TRP2 (white arrow, TRP1), and statistical chart reveals TRP1 expression in the hair follicles after sFRP4 or PBS treatment. (c) Double staining shows colocalization of β-catenin and TRP2 (white arrow, β-catenin), and statistical chart reveals nuclear β-catenin expression in the hair follicles after sFRP4 or PBS treatment. Data are reported as average ± SD. . Bars: 50 um.

(a)

(b)

(c)