Research Article

MicroRNA-214 Suppresses Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Targeting ATF4

Figure 4

miR-214 downregulates ATF4 protein expression. (a) Stable cell line hPDLSCs-miR-214 overexpressing miR-214 was constructed by lentivirus marked with GFP. Representative image of hPDLSCs-miR-214. Scale bar, 100 μm. (b) qPCR analysis was performed to detect miR-214 expression within hPDLSCs-miR-214. (c, d) After 14 days of culture, qRT-PCR and Western blotting were conducted to determine the mRNA and protein levels of ATF4. ; NS: no significance.