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Figure 5: miR-214 negatively regulates osteogenic differentiation of hPDLSCs. (a) ALP activity assay was measured at 7 and 14 days. (b) Alizarin red staining of calcium deposition in hPDLSCs-miR-214 and hPDLSCs-control with or without osteogenic induction (left) and the semiquantitative results (right). (c) Osteogenic marker genes RUNX2, ALP, and OCN were evaluated by qPCR after 7 days osteogenic induction. (d) ATF4 and RUNX2 protein in hPDLSCs-miR-214 and hPDLSCs-control were evaluated by western blot analysis after induction for 7 and 14 days. , , and .