The Impact of GFP Reporter Gene Transduction and Expression on Metabolomics of Placental Mesenchymal Stem Cells Determined by UHPLC-Q/TOF-MS
Table 1
Candidate metabolites with significant changes between the hPMSC and GFP+hPMSC samples detected by isotope labeling liquid chromatography-mass spectrometry (CIL LC-MS) analysis.
(a)
No.
Retention time (s) m/z
∆PG average
†GP average
Candidate name
HMDB umber
Formula
1
200.90
125.0152
1.89
0.47
Taurine
HMDB00251
C2H7NO3S
2
505.02
103.0634
2.02
0.55
Gamma-aminobutyric acid
HMDB00112
C4H9NO2
3
621.05
316.1400
0.69
1.58
Dityrosine
HMDB06045
C18H20N2O6
4
703.73
112.0278
0.42
2.39
Uracil
HMDB00300
C4H4N2O2
5
710.63
327.6295
0.57
1.83
6
780.23
319.6334
0.62
1.51
7
1046.74
380.5105
0.67
1.57
8
1127.03
243.4506
0.61
1.71
9
1156.70
87.1059
0.67
1.60
10
1252.74
101.1210
0.56
1.65
11
1336.96
115.1352
0.59
1.74
DL-2-aminooctanoic acid
HMDB00991
C8H17NO2
(b)
No.
∆PG1-1 ratio
∆PG1-2 ratio
∆PG1-3 ratio
∆PG2-1 ratio
∆PG2-2 ratio
∆PG2-3 ratio
†GP1-1 ratio
†GP1-2 ratio
†GP1-3 ratio
†GP2-1 ratio
†GP2-2 ratio
†GP2-3 ratio
1
3.09
1.33
2.80
1.58
1.47
1.07
0.21
0.56
0.34
0.47
0.57
0.65
2
3.00
1.99
2.62
1.46
1.36
1.71
0.43
0.42
0.56
0.69
0.83
0.40
3
NA
NA
NA
0.83
0.74
0.50
NA
NA
NA
1.33
1.78
1.64
4
NA
NA
NA
0.23
0.56
0.46
NA
NA
NA
3.92
1.47
1.77
5
NA
NA
NA
0.76
0.34
0.62
NA
NA
NA
1.03
3.00
1.45
6
NA
NA
NA
0.95
0.36
0.54
NA
NA
NA
0.75
2.47
1.29
7
NA
NA
NA
0.89
0.42
0.69
NA
NA
NA
1.19
2.28
1.24
8
NA
NA
NA
0.71
0.69
0.43
NA
NA
NA
1.52
1.54
2.09
9
NA
NA
NA
0.98
0.44
0.59
NA
NA
NA
0.83
2.12
1.84
10
NA
NA
NA
0.77
0.45
0.46
NA
NA
NA
1.15
2.09
1.71
11
NA
NA
NA
0.82
0.37
0.58
NA
NA
NA
0.94
2.65
1.64
Metabolites that overlapped with the matching result from the dansyl standard compound library. ∆†List of 11 significant metabolites with correlated responses from the hPMSCs (P) and the GFP+hPMSCs (G) with the different extraction methods (1, 2) in triplicate (−1 to −3). HMDB: human metabolome database.
