Endogenous NSCs in vivo ischemic stroke adult rats
Stereotactic injection
Anti-CD15 antibody SPIONS
Intraventricular SVZ and RMS regions in adult mouse brain following MCAO
Detected day 1–day 8
(i) Proliferation of endogenous NSCs but no migration towards infarction lesion (ii) Findings may be due to short-term follow-up of only 8 days (iii) SPIONs have less imaging sensitivity than MPIOs (iv) Migration to OB along RMS observed after ischemic stroke (v) Introduction of heterologous antibody risk host immunological response (vi) Many other phenotypes undetected
Intraventricular SVZ and RMS regions in adult mouse brain
Detected 1 day after injection, lasted 7 days total
(i) Small size, low artifact (ii) Increased specificity for NSCs (iii) Can track highly active areas such as OB surface binding less likely to affect biological behavior of cells (iv) Introduction of heterologous antibody risks host immunological response (v) Many other phenotypes undetected
Exogenous neural progenitor cells in vivo ischemic stroke adult mice
Intravenous and implantation into hemisphere contralateral to stroke
Fluorescent mesoporous silica-coated superparamagnetic iron oxide nanoparticles
Right hemisphere following MCAO
Detected and analyzed 1–3 days after injection
(i) Cells injected both intracerebrally and intravenously could be seen migrating to ischemic sites of MCAO mice (ii) Migrated cells/cell clusters were detected nearby the lesion boundary (iii) Migrated cells reduced the MR signal intensity in ischemic region 3 days after injection
