Angelica Sinensis Polysaccharide Prevents Hematopoietic Stem Cells Senescence in D-Galactose-Induced Aging Mouse Model

Figure 3

Effect of ASP on mRNA and protein expression of p16-Rb and p53-p21 of Sca-1⁺ HSC/HPCs in D-gal-induced aging mice. The Sca-1+ HSC/HPCs were collected after the treatment. (a) p16 mRNA expression analyses by qRT-PCR. (b) p21 mRNA expression by qRT-PCR. (c) The protein expression analyses of p16, Rb, p53, and p21 by Western blot, β-actin was used as the internal control. (d) The relative protein expression of p16-Rb signaling. (e) The relative protein expression of p53-p21 signaling. Different letters: .

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