A Conditioned Medium of Umbilical Cord Mesenchymal Stem Cells Overexpressing Wnt7a Promotes Wound Repair and Regeneration of Hair Follicles in Mice

Figure 6

Wnt-CM inhibits migration and expression of the cytoskeletal components K6 and K16 via upregulation of c-Myc in human keratinocytes. (a and b) Wnt-CM inhibited migration of human keratinocytes in the wound scratch assay after 24 h as compared to MSC-CM and DMEM. (c) Expression levels of c-Myc mRNA after treatment with Wnt-CM, MSC-CM, or DMEM were analyzed by real-time PCR and normalized to that of GAPDH. (d) Expression levels of K6 and K16 mRNA after treatment with Wnt-CM, MSC-CM, and DMEM were analyzed by RT-PCR and normalized to that of GAPDH. (e, f) Western blots analysis of c-Myc, K6, K16, and corresponding semiquantitative analysis. The data represent mean ± SEM, ; and . Solid lines indicate the initial wound area; dotted lines demarcate the migrating front of the cells. The scale bar in panel (a) is 100 μm.

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