Stem Cells International

Research Article

Canine Adipose Derived Mesenchymal Stem Cells Transcriptome Composition Alterations: A Step towards Standardizing Therapeutic

Table 2

Results of relative gene expression analysis. Summary of all results in different comparison set (CS).
GENE NAMECS1CS2CS3CS4CS5
All donorsY P3/Y P5O P3/O P5Y P3/O P3Y P5/O P5
ABCB1−3,70↓−12,09↓ −2,70↓3,16↑
ACTA2  2,09↑  
CEBPD  −2,32↓  
FGF10  2,23↑−3,05↓−2,50↓
JAG1   2,93↑ 
LPL−2,82↓−2,82↓−2,82↓3,75↑3,02↑
NOTCH    −2,05↓
SOX9   −4,24↓−2,45↓
TBX5   −2,19↓−2,3↓
BDNF   2,10↑ 
BMP72,10↑ 4,76↑−3,98↓ 
CSF3 −2,65↓  2,38↑
EGF  −2,98↓  
FUT12,15↑ 2,55↑  
GTF3A  3,68↑ 2,84↑
HGF −2,44↓2,10↑−68,91↓−16,6↓
IL63,16↑4,59↑2,15↑4↑ 
KITLG    −2,18↓
NES   2,09↑−3,2↓
PTPRC −6,52↓   
ALCAM    −2,65↓
FZD9   −2,36↓2,33↑
MCAM   2,42↑2,08↑
NGFR2,81↑    
NT5E  −2,06↓−3,98↓−6,29↓
VCAM1   −5,37↓−9,5↓
VWF2,48↑2,00↑3,08↑  
CASP3  −2,11↓  
POU5F1  3,97↑  
SOX2  5,07↑  
Total (%)7 (100)7 (100)15 (100)15 (100)16 (100)
Upregulated (%)5 (71,42)2 (28,57)10 (66,66)6 (40)6 (37,5)
Downregulated (%)2 (28,57)5 (71,42)5 (33,33)9 (60)10 (62,5)
Total (%)2 (28,57)2 (28,57)4 (26,6)6 (40)6 (37,5)
Differentiation markers2 (28,57)2 (28,57)4 (26,6)6 (40)6 (37,5)
MSCs associated markers (%)3 (42,8)4 (57,1)6 (40)5 (33,33)5 (31,2)
MSCs specific markers (%)2 (28,57)1 (14,28)3 (20)4 (26,6)5 (31,2)
Stemness markers (%)//2 (13,3)//
CS = comparison set, Y = young donor, O = old donor, and P = passage; numbers in italic and bold indicate fold regulation value.