Research Article

Long Noncoding RNA uc001pwg.1 Is Downregulated in Neointima in Arteriovenous Fistulas and Mediates the Function of Endothelial Cells Derived from Pluripotent Stem Cells

Figure 1

Long noncoding RNA (lncRNA) profiles differentiate the stenosed vein segments of arteriovenous fistulas (AVFs) from the control segments. (a) A volcano plot provided the fold change and P values of differentially expressed lncRNAs. The vertical lines represent a 1.5-fold change in expression (up or down), and the horizontal lines represent P values = 0.05. (b) Heatmap of selected aberrantly expressed lncRNAs in the stenosed vein segments of AVF and the controls. Colors indicate relative signal intensities: red and green colors indicate upregulated and downregulated lncRNAs, respectively. (c) Verification of uc001pwg.1 by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) in the stenosed vein segments of AVF and the controls. (d) qRT-PCR analysis of uc001pwg.1 in different human vessel cells (VSMC: vein smooth muscle cells; EC: endothelial cells; F: fibroblasts). Triplicate assays were done for each RNA sample, and the relative amount of uc001pwg.1 was normalized to GAPDH. Values are expressed as mean ± standard deviation. .
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