TNF-α Induced the Enhanced Apoptosis of Mesenchymal Stem Cells in Ankylosing Spondylitis by Overexpressing TRAIL-R2

Figure 5

The transduction efficiencies of lentiviruses encoding shRNA-TRAIL-R2 were comparable in HDMSCs and ASMSCs. (a) GFP-positive cells were observed under a confocal microscope (40x), and the transduction efficiencies were comparable between HDMSCs and ASMSCs. (b) The lentiviruses encoding shRNA-TRAIL-R2 inhibited both the gene and protein expression of TRAIL-R2 in HDMSCs () and ASMSCs (). Values are presented as the mean ± SD. indicates between HDMSCs and ASMSCs. & indicates for the TNF-α/CHX group compared with other HDMSCs groups. indicates for the TNF-α/CHX group compared with other ASMSCs groups. Lv-NC refers to MSCs transduced by lentiviruses encoding a negative control sequence. Lv-TRAIL-R2 refers to MSCs transduced by lentiviruses encoding TRAIL-R2.

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