Research Article

The Transcription Factor Nrf2 Protects Angiogenic Capacity of Endothelial Colony-Forming Cells in High-Oxygen Radical Stress Conditions

Figure 2

Nrf2 pathway modulation and oxidative stress: (a) intracellular ROS levels as measured by CM-H2DCFDA. ECFCs show a dose-dependent increase in CM-H2DCFDA signal in response to H2O2 (). Keap1 knockdown reduces oxidative stress in the presence of higher concentrations of H2O2 (), whereas Nrf2 knockdown increases susceptibility to higher concentrations of H2O2 (). Graphs represent mean +/− SEM, and data are from 3 independent biological replicates.