Human Suprapatellar Fat Pad-Derived Mesenchymal Stem Cells Induce Chondrogenesis and Cartilage Repair in a Model of Severe Osteoarthritis

Figure 2

Supra- and infrapatellar ASC proliferative activity. (a) Colony-forming unit (CFU) assay was performed from infra and suprapatellar SVF from four different patients in the presence of 10% human serum (HS) or 10% fetal bovine serum- (FBS-) containing medium for 10 days. The generated colonies were counted after Giemsa staining (right panel). The mean ± SD of the total number of colonies at each condition of all tested samples was shown. at the indicated comparisons. (b) 10⁴ cells at passage three of every sample, suprapatellar (blue line)- and infrapatellar (red line)-derived ASC were allowed to grow in multiple wells in the presence of 10% HS or 10% FBS and quantified every day up to 10 days. The mean ± SD of the total number of cells at every time point was represented. versus infrapatellar. (c) Representative images after TEM acquisition of suprapatellar ASC growth in HS (left panels) or FBS (right panels) are shown. White arrows: inclusion bodies; black arrows: intermediate filaments.

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