Evaluating Wharton’s Jelly-Derived Mesenchymal Stem Cell’s Survival, Migration, and Expression of Wound Repair Markers under Conditions of Ischemia-Like Stress

Table 1

(a) Primer sequences used for semiquantitative RT-PCR analysis

S. number	Gene name	Forward primer	Reverse primer	Size (bp)
1	IGF-1	5′ TCTTGAAGGTGAAGATGCACACCA 3′	5′ AGCGAGCTGACTTGGCAGGCTTGA 3′	303
2	COL14A1	5′ AAGCCCAGAGTCAAAGTTGTGGA 3′	5′ CCATGAACCATCGACCAGGA 3′	123
3	MMP 2	5′ GGCCCTGTCACTCCTGAGAT 3′	5′ GGCATCCAGGTTATCGGGGA 3′	473
4	ITGA1	5′ GGTTCCTACTTTGGCAGTATT 3′	5′ AACCTTGTCTGATTGAGAGCA 3′	149
5	ITGA6	5′ TCCCTGAACCTAACGGAGTCT 3′	5′ ATGTCCAAGTAGTTCAGTTTG 3′	253
6	SDF-1	5′ ATGAACGCCAAGGTCGTGGTC 3′	5′ CTTGTTTAAAGCTTTCTCCAGGTACT 3′	267
7	WISP-1	5′ AGAGCCGCCTCTGCAACTT 3′	5′ GGAGAAGCCAAGCCCATCA 3′	245
8	GAPDH	5′ GAGTCAACGGATTTGGTCGT 3′	5′ TTGATTTTGGAGGGATCTCG 3′	248
9	VTN	5′ CGAGGAGAAAAACAATGCCAC 3′	5′ GAAGCCGTCAGAGATATTTCG 3′	498
10	TSG 6	5′ CCCATTGTGAAGCCAGGGCCCAACTG 3′	5′ GGAAGCTCATCTCCACAGTATCTTCCC 3′	361
11	HGF	5′ TCACGAGCATGACATGACTCC 3′	5′ AGCTTACTTGCATCTGGTTCC 3′	302

(b) Human wound healing-related genes screened using the RT² Profiler PCR Array

ACTA2	ACTC1	ANGPT1	CCL2	CCL7	CD40LG	CDH1	COL14A1	COL1A1	COL1A2	COL3A1	COL4A1
COL4A3	COL5A1	COL5A2	COL5A3	CSF2	CSF3	CTGF	CTNNB1	CTSG	CTSK	CTSV	CXCL1
CXCL11	CXCL2	CXCL5	FGF	EGFR	F13A1	F3	FGA	FGF10	FGF2	FGF7	HBEGF
HGF	IFN-G	IGF1	IL10	IL1B	IL2	IL4	IL6	IL6ST	ITGA1	ITGA2	ITGA3
ITGA-4	ITGA-5	ITGA-6	ITGAV	ITGB1	ITGB3	ITGB5	ITGB6	MAPK1	MAPK3	MIF	MMP1
MMP2	MMP7	MMP9	PDGFA	PLAT	PLAU	PLAUR	PLG	PTEN	PTGS2	RAC1	RHOA
SERPINE1	STAT3	TAGLN	TGFA	TGFB1	TGFBR3	TIMP1	TNF	VEGFA	VTN	WISP1	WNT5A

keeping genes. genomic DNA contamination control.
transcription control. PCR control.