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Stem Cells International
Volume 2017, Article ID 5270527, 8 pages
https://doi.org/10.1155/2017/5270527
Research Article

Paracrine Effects of Bone Marrow Mononuclear Cells in Survival and Cytokine Expression after 90% Partial Hepatectomy

1Experimental Laboratory of Gastroenterology and Hepatology, HCPA, Ramiro Barcelos 2350, 90035-903 Porto Alegre, RS, Brazil
2Post-Graduation Program on Medicine: Sciences in Gastroenterology, UFRGS, Ramiro Barcelos 2350, 90035-903 Porto Alegre, RS, Brazil
3Gene Therapy Center, HCPA, Ramiro Barcelos 2350, 90035-903 Porto Alegre, RS, Brazil
4Post-Graduation Program on Genetics and Molecular Biology, UFRGS, Av. Bento Gonçalves 9500, 91501-970 Porto Alegre, RS, Brazil
5Post-Graduation Program in Surgery, UFRGS, Ramiro Barcelos 2350, 90035-903 Porto Alegre, RS, Brazil
6Post-Graduation Program in Child and Adolescent Health, UFRGS, Ramiro Barcelos 2350, 90035-903 Porto Alegre, RS, Brazil

Correspondence should be addressed to Ursula Matte; rb.ude.apch@ettamu

Received 23 September 2016; Revised 30 November 2016; Accepted 10 January 2017; Published 23 February 2017

Academic Editor: Dario Coletti

Copyright © 2017 Carlos Oscar Kieling et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Acute liver failure is a complex and fatal disease. Cell-based therapies are a promising alternative therapeutic approach for liver failure due to relatively simple technique and lower cost. The use of semipermeable microcapsules has become an interesting tool for evaluating paracrine effects in vivo. In this study, we aimed to assess the paracrine effects of bone marrow mononuclear cells (BMMC) encapsulated in sodium alginate to treat acute liver failure in an animal model of 90% partial hepatectomy (90% PH). Encapsulated BMMC were able to increase 10-day survival without enhancing liver regeneration markers. Gene expression of Il-6 and Il-10 in the remnant liver was markedly reduced at 6 h after 90% PH in animals receiving encapsulated BMMC compared to controls. This difference, however, was neither reflected by changes in the number of CD68+ cells nor by serum levels of IL6. On the other hand, treated animals presented increased caspase activity and gene expression in the liver. Taken together, these results suggest that BMMC regulate immune response and promote apoptosis in the liver after 90% PH by paracrine factors. These changes ultimately may be related to the higher survival observed in treated animals, suggesting that BMMC may be a promising alternative to treat acute liver failure.