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Figure 1: miR-326 and βarr-1 are downregulated in cerebellar NSCs compared to the bulk cerebellar population. (a) mRNA expression levels of the primary (pri-miR-326) and precursor (pre-miR-326) of miR-326 in NSCs compared to bulk cells (T0). (b) Mature miR-326 levels in NSCs compared to bulk cells (T0). (c) βarr-1 mRNA levels in NSCs compared to bulk cells (T0). (d) Western Blot (WB) analysis of endogenous βarr-1 and Nanog in NSCs compared to bulk cells (T0). Actin as loading control (LC). (a)–(d) data are means ± SD from 3 independent experiments. .