Research Article

Stemness Maintenance Properties in Human Oral Stem Cells after Long-Term Passage

Figure 4

Characterization of early and late passages. (a) qRT-PCR for p16 and p21. The relative fold changes represent differences in gene expression at P15 compared to P2 in hPDLSCs. p16 and p21 gene expressions showed a statistically significant difference at passage 15 (). (d) p16 and p21 protein expressions at P2 and P15 hPDLSCs were validated by means of Western blotting analysis. (b) The relative fold changes represent differences in gene expression at P15 compared to P2 in hDPSCs. p16 and p21 gene expressions showed a statistically significant difference at passage 15 when compared to P2 (). (e) Western blotting analysis of p16 and p21 validated gene expression profile at P2 and P15 of hDPSCs. (c) The relative fold changes represent differences in gene expression at P15 compared to P2 in hGMSCs. p16 and p21 gene expressions showed a statistical significance at passage 15 (). (f) Western blotting analysis of p16 and p21 at P2 and P15 hGMSCs confirmed gene expression analyses. (g, h, and i) Densitometric analysis of bands related to p16 and p21 at P2 and P15 in hPDLSCs, hDPSCs, and hGMSCs (). Statistical analyses were performed using one-way ANOVA and Bonferroni post hoc test.