Stemness Maintenance Properties in Human Oral Stem Cells after Long-Term Passage
Table 1
Cytofluorimetric analysis of hPDLSCs.
Flow cytometry phenotyping of hPDLSCs
Antigen
Phenotype
hPDLSC MFI
P2
P15
value
CD13
+++
172.4 ± 38.1
157.6 ± 42.5
0.456
CD14
−
1.1 ± 0.2
1.2 ± 0.2
0.104
CD29
+++
198.6 ± 53.1
143.6 ± 43.2
0.099
CD31
−
1.3 ± 0.3
1.2 ± 0.1
0.462
CD34
−
1.2 ± 0.2
1.1 ± 0.1
0.306
CD44
+++
166.7 ± 32.6
124.1 ± 33.9
0.107
CD45
−
1.3 ± 0.1
1.1 ± 0.1
0.193
CD73
+
29.2 ± 8.9
22.3 ± 5.9
0.169
CD90
+++
396.1 ± 42.4
216.3 ± 37.3
↓0.016
CD105
+
9.1 ± 3.4
5.5 ± 1.3
0.134
CD117
−
1.1 ± 0.2
1.2 ± 0.2
0.349
CD133
−
1.2 ± 0.3
1.3 ± 0.3
0.347
CD166
+
18.7 ± 8.5
20.7 ± 8.2
0.439
CD326
−
1.3 ± 0.2
1.4 ± 0.1
0.310
HLA-ABC
++
127.1 ± 48.1
93.9 ± 21.1
0.283
HLA-DR
−
1.2 ± 0.1
1.4 ± 0.1
0.396
NANOG
+
7.7 ± 2.2
8.6 ± 1.7
0.127
OCT4
+
3.1 ± 0.4
3.1 ± 0.5
0.429
SSEA4
+
4.7 ± 1.6
4.9 ± 1.2
0.204
SOX2
+
60.1 ± 23.3
66.4 ± 18.4
0.394
− indicates negative expression (0%); + indicates moderate expression; ++ indicates positive; +++ indicates high expression (100%); MFI ratio is the average of five different biological ; bold values represent MFI ratio with ; cutoff MFI ratio .
