Stemness Maintenance Properties in Human Oral Stem Cells after Long-Term Passage
Table 2
Cytofluorimetric analysis of hDPSCs.
Flow cytometry phenotyping of hDPSCs
Antigen
Phenotype
hDPSC MFI
P2
P15
value
CD13
++
140.3 ± 37.7
145.1 ± 15.9
0.425
CD14
−
1.2 ± 0.2
1.3 ± 0.2
0.260
CD29
+++
235.7 ± 24.8
150.2 ± 27.5
↓0.022
CD31
−
1.5 ± 0.3
1.7 ± 0.1
0.153
CD34
−
1.2 ± 0.2
1.4 ± 0.1
0.208
CD44
++
92.1 ± 21.1
99.8 ± 24.3
0.446
CD45
−
1.2 ± 0.1
1.1 ± 0.1
0.463
CD73
++
58.3 ± 8.9
44.9 ± 6.6
0.075
CD90
+++
221.7 ± 39.7
411.5 ± 36.7
↑0.007
CD105
+
8.4 ± 3.1
6.2 ± 1.9
0.204
CD117
−
1.3 ± 0.2
1.4 ± 0.2
0.198
CD133
−
1.7 ± 0.3
1.3 ± 0.3
0.401
CD166
+
11.1 ± 4.8
17.9 ± 3.3
0.055
CD326
−
1.1 ± 0.2
1.1 ± 0.1
0.287
HLA-ABC
++
84.7 ± 6.8
79.6 ± 8.2
0.246
HLA-DR
−
1.2 ± 0.1
1.4 ± 0.1
0.399
NANOG
+
5.9 ± 2.5
6.9 ± 2.4
0.261
OCT4
+
2.5 ± 0.3
2.5 ± 0.4
0.171
SSEA4
+
4.6 ± 0.8
4.1 ± 0.6
0.377
SOX2
+
44.9 ± 6.8
45.1 ± 8.8
0.119
− indicates negative expression (0%); + indicates moderate expression; ++ indicates positive; +++ indicates high expression (100%); MFI ratio is the average of five different biological ; bold values represent MFI ratio with ; cutoff MFI ratio .
