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Stem Cells International
Volume 2017, Article ID 5946527, 11 pages
Research Article

Plasma Rich in Growth Factors Induces Cell Proliferation, Migration, Differentiation, and Cell Survival of Adipose-Derived Stem Cells

1Tissue and Neuronal Regeneration Laboratory, Centro de Investigación Príncipe Felipe, Valencia, Spain
2Unidad de Artroscopia y Unidad de Traumatología del Hospital Quiron, Barcelona, Spain
3Fundación García Cugat, Barcelona, Spain
4FactorStem Ltd., Valencia, Spain

Correspondence should be addressed to Victoria Moreno-Manzano; se.fpic@moneromv

Received 30 May 2017; Revised 25 July 2017; Accepted 5 September 2017; Published 15 November 2017

Academic Editor: Xuekun Li

Copyright © 2017 Maravillas Mellado-López et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Adipose-derived stem cells (ASCs) are a promising therapeutic alternative for tissue repair in various clinical applications. However, restrictive cell survival, differential tissue integration, and undirected cell differentiation after transplantation in a hostile microenvironment are complications that require refinement. Plasma rich in growth factors (PRGF) from platelet-rich plasma favors human and canine ASC survival, proliferation, and delaying human ASC senescence and autophagocytosis in comparison with serum-containing cultures. In addition, canine and human-derived ASCs efficiently differentiate into osteocytes, adipocytes, or chondrocytes in the presence of PRGF. PRGF treatment induces phosphorylation of AKT preventing ASC death induced by lethal concentrations of hydrogen peroxide. Indeed, AKT inhibition abolished the PRGF apoptosis prevention in ASC exposed to 100 μM of hydrogen peroxide. Here, we show that canine ASCs respond to PRGF stimulus similarly to the human cells regarding cell survival and differentiation postulating the use of dogs as a suitable translational model. Overall, PRGF would be employed as a serum substitute for mesenchymal stem cell amplification to improve cell differentiation and as a preconditioning agent to prevent oxidative cell death.