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Stem Cells International
Volume 2017, Article ID 5946527, 11 pages
Research Article

Plasma Rich in Growth Factors Induces Cell Proliferation, Migration, Differentiation, and Cell Survival of Adipose-Derived Stem Cells

1Tissue and Neuronal Regeneration Laboratory, Centro de Investigación Príncipe Felipe, Valencia, Spain
2Unidad de Artroscopia y Unidad de Traumatología del Hospital Quiron, Barcelona, Spain
3Fundación García Cugat, Barcelona, Spain
4FactorStem Ltd., Valencia, Spain

Correspondence should be addressed to Victoria Moreno-Manzano; se.fpic@moneromv

Received 30 May 2017; Revised 25 July 2017; Accepted 5 September 2017; Published 15 November 2017

Academic Editor: Xuekun Li

Copyright © 2017 Maravillas Mellado-López et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1 Canine ASC were cell cultured with FBS or canine PRGF at growing concentration (1, 2.5, 5 or 10%) or in the absent of growth factors (0) for 24 hours and subjected to cell viability test analysis. 10% PRGF induced a significant difference on the cell numbers in comparison with 10% of FBS; *p>0.05 vs 0%, $p>0.05 vs 10% FBS. Supplementary Figure 2 Canine ASC were induced to differentiate toward the three mesodermal lineages in the presence or absence of canine 2.5% PRGF. A) Adipogenesis was evidenced by intracellular lipid content by Oil Red O staining; B) Osteogenesis was detected by calcium deposits visualized by Alizarin Red staining and phalloidin (green) and Cx43 expression (red); C) Chondrogenesis was analysed by Alcian blue staining (marker of the proteoglycan aggrecan deposits), and immunostaining of Sox9 (green; marker of chondrogenesis and chondrocyte differentiation).

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