Effect of Uniaxial Tensile Cyclic Loading Regimes on Matrix Organization and Tenogenic Differentiation of Adipose-Derived Stem Cells Encapsulated within 3D Collagen Scaffolds

Figure 9

Effect of uniaxial tensile loading on myogenic gene expression of ASCs within 3D collagen scaffolds. Gene expression profiles of ASCs encapsulated within collagen scaffolds subjected to BMP-12 treatment or uniaxial loading at 0% (unloaded), 2%, 4%, and 6% strains at 0.1 Hz and 1 Hz loading frequencies. The graphs depict fold changes in various myogenic markers: MyoD and myogenin. No significant increase of myogenic markers in ASCs was observed in any of the mechanically stimulated groups.