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Stem Cells International
Volume 2017, Article ID 6082684, 11 pages
Research Article

hTERT-Immortalized Bone Mesenchymal Stromal Cells Expressing Rat Galanin via a Single Tetracycline-Inducible Lentivirus System

1Department of Anesthesiology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China
2Department of Anesthesiology, The Third Xiangya Hospital of Central South University, Changsha 410013, China
3Laboratory of Research Center for Translational Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China
4Department of Anesthesiology, The First Affiliated Hospital of Shenzhen University, Shenzhen 518035, China

Correspondence should be addressed to Ke An; moc.621@hw2002ekna

Received 21 November 2016; Revised 26 January 2017; Accepted 22 February 2017; Published 11 May 2017

Academic Editor: Veronica Ramos-Mejia

Copyright © 2017 Ke An et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The use of human telomerase reverse transcriptase-immortalized bone marrow mesenchymal stromal cells (hTERT-BMSCs) as vehicles to deliver antinociceptive galanin (GAL) molecules into pain-processing centers represents a novel cell therapy strategy for pain management. Here, an hTERT-BMSCs/Tet-on/GAL cell line was constructed using a single Tet-on-inducible lentivirus system, and subsequent experiments demonstrated that the secretion of rat GAL from hTERT-BMSCs/Tet-on/GAL was switched on and off under the control of an inducer in a dose-dependent manner. The construction of this cell line is the first promising step in the regulation of GAL secretion from hTERT-immortalized BMSCs, and the potential application of this system may provide a stem cell-based research platform for pain.