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Stem Cells International
Volume 2017, Article ID 6129596, 10 pages
Research Article

Predicting the Remaining Lifespan and Cultivation-Related Loss of Osteogenic Capacity of Bone Marrow Multipotential Stromal Cells Applicable across a Broad Donor Age Range

1Leeds Institute of Rheumatic and Musculoskeletal Medicine, University of Leeds, Beckett Street, Leeds LS9 7TF, UK
2NIHR Research Unit, Chapel Allerton Hospital, Leeds Teaching Hospital NHS Trust, Chapeltown Road, Leeds LS7 4SA, UK
3School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK

Correspondence should be addressed to Elena A. Jones;

Received 25 November 2016; Accepted 16 January 2017; Published 19 February 2017

Academic Editor: Krzysztof Marycz

Copyright © 2017 Sarah M. Churchman et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The supplementary document contains evidence of (1) the upregulation of genes between passage 0 and senescence, (2) the effect of culture expansion upon chondrogenic genes and (3) the effect of osteogenic induction on selected genes. Furthermore, in support of developing the predictive model, the evidence for selection of the candidate molecules is shown (4), with their robustness in alternative medium (5). The predictive model was further tested excluding the more diverse-in-age donors (6).

  1. Supplementary Material