Y-box Protein-1 Regulates the Expression of Collagen I in Hepatic Progenitor Cells via PDGFR-β/ERK/p90RSK Signalling

Figure 5

YB-1 regulates collagen I expression via PDGFR-β/ERK/p90RSK signalling. (a) YB-1 silencing enhanced collagen I expression in HPCs, but the PDGFR-β inhibitor DMPQ blunted the regulatory effect of YB-1 silencing on collagen I expression. (b) Seven days after YB-1 knockdown, HPCs were harvested to analyse the signalling pathway involved in YB-1-regulated collagen I expression. Following YB-1 silencing, phosphorylated YB-1 also decreased, while PDGFR-β and phosphorylated ERK1/2 were elevated. The level of phosphorylated Stat3 and PI3K showed no alteration. (c) The ERK1/2 inhibitor FR180204 repressed phosphorylation of p90RSK1 and ERK1/2 and abolished YB-1-regulated collagen I expression.

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