Cytokine, Chemokine, and Growth Factor Profile Characterization of Undifferentiated and Osteoinduced Human Adipose-Derived Stem Cells

Figure 5

Calcium quantification. Cell calcium content was determined kit recurring to a colorimetric assay. Evaluation was performed at day 14 for SaOs-2 and at day 21 for MG-63 and ASCs. For each cell type, data were normalized on control condition (CM) set as 1. OM condition significantly increases the calcium content in each cell type with a particularly high level in SaOs-2. CM = control medium (DMEM 2% FBS); OM = osteogenic medium (see Materials and Methods). Statistical analysis was performed by using the Wilcoxon–Mann–Whitney test. A value of <0.05 was considered significant.