Xeno-Free Strategies for Safe Human Mesenchymal Stem/Stromal Cell Expansion: Supplements and Coatings

Table 4

Commercially available coating strategies for hMSC in vitro culture.

Name	Company	Composition	Validation (cell types)
Soluble coatings
CELLstart™	Thermo Fisher	Not disclosed	hMSC, human embryonic stem cells (hESC), and human neural stem cells (hNSC)
Nutristem MSC attachment solution	Biological industries	Affinity-purified human plasma fibronectin	BM-hMSC, AT-hMSC, UC-hMSC
Xuri MSC attachment solution	GE Healthcare	Affinity-purified human plasma fibronectin	Validated for human BM-hMSC, AT-hMSC, and UC-hMSC
Modified plasticware
BD PureCoat™ ECM Mimetic Cultureware: collagen and fibronectin	Corning	Synthetic animal-free peptides covalently linked to a proprietary surface: (i) fibronectin-derived RGD peptide; (ii) collagen I-derived peptide	BM-hMSC and AT-hMSC; human umbilical cord blood-derived MSC (isolation)
Corning® Synthemax® Surface	Corning	Peptide acrylate coating functionalized with a vitronectin-derived peptide	Validated for hPSCs and other adult stem cell types