| | Medium | Basal media | Serum | Coating/coating-free
supplements | Supplementation |
| | Expansion FBS | DMEM/F-12
(Life Technologies, Gibco,
Rockville, MD) | 10% FBS
(Life Technologies) | None | 1% GlutaMAX
(GlutaMAX I; Life Technologies),
1% p/s (p/s; 100 U/mL penicillin,
0.1 mg/mL streptomycin; Lonza) | | Expansion HS | DMEM/F-12 | 10% HS (Lonza,
Walkersville, MD) | None | 1% GlutaMAX, 1% p/s | | Expansion XF/SF | StemPro MSC SFM
(Life Technologies) | None | CELLstart™ coating
(Life Technologies) | 1% GlutaMAX, 0,3% p/s,
StemPro MSC SFM XenoFree
supplement (Life Technologies) | Adipogenic
FBS/HS | DMEM/F-12 | 10% FBS/HS | None | For 4 days induction during cyclic
differentiation: 1% GlutaMAX,
1% p/s, 0.5 mM isobutylmethylxanthine
(IBMX; Sigma), 0.2 mM indomethacin
(Sigma), 1 μM dexamethasone (Sigma),
and 10 μg/mL insulin (Sigma) | Adipogenic
XF/SF | StemPro
MSC SFM | None | CELLstart coating | For 4 days induction during cyclic
differentiation: 1% GlutaMAX, 0,3% p/s,
StemPro MSC SFM XenoFree supplement,
same adipogenic supplements as
in FBS/HS cultures | Osteogenic
FBS/HS | DMEM/F-12 | 10% FBS/HS | None | 1% GlutaMAX, 1% p/s,
150 μM L-ascorbic acid
2-phosphate (Sigma),
10 mM beta-glycerophosphate (Sigma),
10 nM dexamethasone (Sigma) | Ostegenic
XF/SF | StemPro
MSC SFM | None | CELLstart coating | 1% GlutaMAX, 0,3% p/s,
StemPro MSC SFM XenoFree supplement,
same osteogenic supplements as
in FBS/HS cultures | Chondrogenic
FBS/HS | DMEM/F-12 | None | None | 1% GlutaMAX, 0,2% p/s,
1x ITS + 1 (Sigma), 50 μM L-ascorbic acid
2-phosphate, 55 μM sodium pyruvate
(Life Technologies), 23 μM L-proline (Sigma),
10 ng/mL TGF-β (Sigma) | Chondrogenic
XF/SF | StemPro
MSC SFM | None | None | 1% GlutaMAX, 0,2% p/s, StemPro
MSC SFM XenoFree supplement,
same chondrogenic supplements as
in FBS/HS cultures |
|
|
