BMP4/LIF or RA/Forskolin Suppresses the Proliferation of Neural Stem Cells Derived from Adult Monkey Brain

Figure 1

A flow chart of a method used in neural stem cell-like cell isolation from the monkey brain (Macaca fascicularis). (a) Primary cells were obtained from the cultured monkey brain. (b) The detail of cell-isolating process was described. The monkey brain cortex was treated with enzyme and cultured for two months. Within these months, fresh-specific medium was changed to neural stem cells (cell sedimentation after centrifugation) every two days. (c) Monkey neurospheres occurred, and neural stem cell-like cells proliferated from neurospheres. (A) Monkey neurospheres appeared in adult monkey brain culture medium. (B) Neural stem cell-like cells proliferated from neurospheres. (C) Large number of neural stem cell-like cells formed an independent clone, and this clone was seen at low-magnification microscope. (D) The proliferating rate of monkey NSC-like cells was shown. Scale bar = 50 μm.