Tissue Source and Cell Expansion Condition Influence Phenotypic Changes of Adipose-Derived Stem Cells
Assessment of lipid content of differentiated and undifferentiated ASCs. Triglyceride content and Oil red O were assessed in cells differentiated in media supplemented with 10% FBS or 10% hPL and normalized to total cellular protein content. Data are expressed as triglyceride content (mg/mg protein) in undifferentiated (CTRL) and differentiated (adipogenic) cells (a) and as fold change following adipogenic differentiation (b). Oil red O content is expressed as a relative fold change following adipogenic differentiation (c). Statistically significant differences were determined by one-way ANOVA with Tukey’s multiple comparisons. Data represents the mean ± SEM of three biological replicates from three donors/group. RB represents the mean ± SEM of three biological replicates from one donor from a commercial source. Significant differences between groups are designated by alphabetical letters. Any groups with the same letter are not statistically different from each other (, Tukey’s MCT).
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