Kindlin-2 Modulates the Survival, Differentiation, and Migration of Induced Pluripotent Cell-Derived Mesenchymal Stromal Cells
Kindlin-2 regulates apoptosis and stem cell markers in iPSC-MSCs. The iPSC-MSCs were stained using annexin V and propidium iodide for analyzing cell apoptosis after transfection with Kindlin-2 constructs and quantified for early and late apoptotic cells. Kindlin-2 knockdown significantly increased apoptotic cells while Kindlin-2 overexpression significantly decreased apoptotic cells compared to control Flag (a). Data were presented as the mean ± SEM from three independent experiments. Dot plots showing single experiments for annexin V/propidium iodide double positive cells for late apoptotic cells (b). (c) and (d) show quantitative RNA expression of the MSC stemness markers CD73 and CD105 in Kindlin-2 overexpressing and knockdown cells and their controls, respectively. Numbers in (b) indicate % cells in quadrant gate. difference .
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