Research Article

Kindlin-2 Modulates the Survival, Differentiation, and Migration of Induced Pluripotent Cell-Derived Mesenchymal Stromal Cells

Figure 4

Differentiation capacity of iPSC-MSCs after Kindlin-2 transfection. Osteogenic, chondrogenic, and adipogenic differentiation potential of hiPSC-MSCs after transfection with constructs modulating Kindlin-2. Alizarin Red staining for mineralized deposits (a), Alcian Blue staining for chondrocyte pellets (b), and Oil Red-O staining for lipid formation (c) formed by the three iPSC-MSC cell lines. The mRNA expression level of the relative expression of genes associated with osteogenesis (osteocalcin and alkaline phosphatase), chondrogenesis (collagen type II and aggrecan), and adipogenesis (PPAR-γ and LPL). The data represent the mean expression values normalized to the housekeeping gene GAPDH. Magnification is 10x. difference .