Research Article

Kindlin-2 Modulates the Survival, Differentiation, and Migration of Induced Pluripotent Cell-Derived Mesenchymal Stromal Cells

Figure 6

Kindlin-2 affects migration potential of iPSC-MSCs. An in vitro migration assay was performed with an IBIDI culture insert containing two reservoirs separated by a 500 μm thick wall. Kindlin-2 Flag transfected iPS-MSCs had significantly increased migration potential after 24 h and 36 h compared to control groups (a). Moreover, the migration assay with a transwell system showed significantly increased migrating iPSC-MSCs on the lower face of the filters after 48 h of incubation in Kindlin-2 Flag transfected iPSC-MSCs compared to control Flag (b). The covered area between the two reservoirs was analyzed with ImageJ software. difference . .